In Situ Hybridization Protocols

In Situ Hybridization Protocols

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Ian Darby updates the highly successful premier edition with a full panoply of new and greatly augmented state-of-the-art techniques. This valuable new edition contains basic and advanced in situ hybridization techniques -- many not covered in the first edition -- and includes protocols for in situ hybridization of whole-mount embryo specimens, in situ hybridization at the electron microscope level, in situ detection of DNA fragmentation in apoptosis, localization of genes to particular chromosomes, and the use of DNA or RNA probes to detect expression in cells or tissue sections. Prepared by leading investigators who have daily worked with the techniques, fine-tuning them for reliability, In Situ Hybridization Protocols, 2nd Edition, offers a completely updated and extended collection of proven and readily reproducible methods suitable for both the novice and experienced investigator. Its state-of-the-art protocols constitute the new standard methods resource in the field.RNase (e.g., Sigma pancreatic RNase A, Code R 5125), stock aliquots of 10 mg/ mL in 10 mM Tris-HCl, 15 mM NaCl, pH 7.5, ... The diluted solution can be frozen and thawed approx 10 times for repeated use. ... Long-wavelength UV from a Sylvania Blacklite-blue, 15 W tube, 350 nm. 3. ... Approx 0.01 M phosphate buffer, pH 6.8, from tablets (BDH tablets, Code 33199 2P), dissolve one tablet per liter of anbsp;...

Title:In Situ Hybridization Protocols
Author: Ian A. Darby
Publisher:Springer Science & Business Media - 2000

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